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. 2016 Mar 22;24(5):946–955. doi: 10.1038/mt.2016.39

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Involvement of AGO2 during inhibition of SIRT1 and ARHGAP1 expression by single-stranded and double-stranded miR-34a mimics. (a) Western blot data showing involvement of AGO2 in SIRT1 inhibition by miR-34a mimics. Data are representative from three independent experiments. (b) qPCR data showing involvement of AGO2 in ARHGAP1 inhibition by miR-34a mimics. (c) qPCR data confirming that AGO1 mRNA levels were reduced after the double transfection with anti-AGO1 siRNA. (d) qPCR data conforming that AGO2 mRNA levels were reduced after the double transfection with anti-AGO2 siRNA. ds-Ctrl, siAGO1, or siAGO2 was transfected into HeLa cells at 25 nmol/l (Day 0). Two days after the first transfection, cell was treated with ss-Ctrl1, modified ss-miR-34a-5p, or unmodified ds-miR-34a(fc) (second transfection, 50 nmol/l) (Day 2) and then harvested for qPCR (Day 4) and western blot (Day 5) analysis. Error bars in panel b–d are mean ± SD. n = 3. *P < 0.05; **P < 0.01; ***P < 0.001 (relative to ds-Ctrl/ss-Ctrl1, unpaired t-test).