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Medium for in vitro cultivation of two-cell mouse ova.
| Concentration |
ml of isotonic stock solution |
|||
|---|---|---|---|---|
| Number | Component | g/l | mM | |
| 1 | NaCl | 6.384 | 109.23 | 9.15 |
| 2 | KC1 | 0.356 | 4.78 | 0.40 |
| 3 | CaCI2 | 0.189 | 1.71 | 0.20 |
| 4 | KH2PO4 | 0.162 | 1.19 | 0.10 |
| 5 | MgSO4 · 7H2O | 0.294 | 1.19 | 0.10 |
| 6 | NaHCO3 | 2.106 | 25.07 | 2.10 |
| 7 | Sodium lactate | 1.147 | 10.15 | 0.85 |
| 8 | Crystalline bovine albumin |
1.000 | — | — |
| 9 | Penicillin | 100 U/ml | — | — |
| 10 | Streptomycin | 50 μg/ml | — | — |
