(A) Expression of YAP1, TAZ, and TEAD1 and phosphorylation of YAP1 were assessed in HCC (SK-Hep1 and SNU-449) and normal hepatocyte cell line (HepaRG) by Western blotting with indicated antibodies.
(B) CTGF mRNA expression was measured by quantitative RT-PCR.
(C) SK-Hep1 and SNU-449 HCC cells were transiently transfected with indicated siRNAs, and cell proliferation rates were measured by MTT assay at the indicated time points. Values shown were normalized to luciferase-specific siRNA (siLuc)-treated cells and represent mean ± standard deviation.