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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Hepatology. 2015 Nov 26;63(1):159–172. doi: 10.1002/hep.28223

Figure 3. Glutamine is necessary for growth of YAP1/TAZ-active HCC cells.

Figure 3

(A) Growth of HCC cells under conditions of glutamine abundance or deprivation. Data are the mean ± standard deviations of three independent cell cultures grown in the presence or absence of glutamine for 48 hours. *** P < 0.001 by Student t-test.

(B) SK-Hep1 and SNU-449 cells were transiently transfected with indicated siRNA for 72 hours. The medium was assayed for glutamine level. The data were normalized to cell number. *p < 0.05, **p < 0.01, ***p < 0.005 by Student t-test. ns: not significant.

(C,D) Consumed glutamine level from the medium after transfection of indicated siRNAs (C) or after expression of exogenous SLC38A1 in YAP1/TAZ-depleted SK-Hep1 cells (D). Cells were transfected with indicated siRNAs or expression vectors for 48 hours. The data were normalized to number of cells. *P < 0.05, **P < 0.01, ***P < 0.005 by Student t-test.