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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Hepatology. 2015 Nov 26;63(1):159–172. doi: 10.1002/hep.28223

Figure 4. Amino acid transporters are important for proliferation of HCC cells.

Figure 4

(A–D) SK-Hep1 and SNU-449 HCC cells were transiently transfected with indicated siRNAs, and cell proliferation rates were measured by MTT assay at the indicated time points. Values shown were normalized to siLuc-treated cells and represent mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.005 by Student t-test.

(E) Recovered cell growth by exogenous SLC38A1 and SLC7A5 in YAP1/TAZ-depleted SK-Hep1 cells. Myc-tagged exogenous SLC38A1 and SLC7A5 were expressed in SK-Hep1 cells. Empty expression vector pCMV6 was used as a control. Cell proliferation rates were measured by MTT assay at 72 hours after transfection of expression vectors. *P < 0.05, **P < 0.01, ***P < 0.005 by Student t-test.

(F,G) Tumor weight after treatment with siRNA-DOPC in a subcutaneous xenograft model (F) or orthotopic xenograft model (G) with SK-Hep1 HCC cells. At 6 weeks after siRNA-DOPC injection, mice were killed and tumor weights measured (n = 10 per group) Data are presented as mean ±standard error of the mean (SEM). *P < 0.05, **P < 0.01, ***P < 0.005 by Student t-test.