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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Hepatology. 2015 Nov 26;63(1):159–172. doi: 10.1002/hep.28223

Figure 6. mTORC1 is regulated by SLC38A1 and SLC7A5 in HCC cells.

Figure 6

(A) SK-Hep1 and SNU-449 HCC cells were transiently transfected with indicated siRNAs for 96 hours. Phosphorylation of S6K1 was assessed by Western blotting with indicated antibodies.

(B) SK-Hep1 cells were treated with esterified glutamine (Gln) (4 mM) for 30 minutes after silencing of SLC38A1 with specific siRNA. Expression and phosphorylation of S6K1 and S6 were assessed in cell lysates by Western blotting with indicated antibodies.

(C,D) Xenografted HCC tissues treated with indicated siRNAs were used for Western blot analysis with indicated antibodies.