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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Hepatology. 2015 Nov 26;63(1):159–172. doi: 10.1002/hep.28223

Figure 7. YAP1 and TAZ activate mTORC1 through regulation of SLC38A1 and SLC7A5.

Figure 7

(A–B) SK-Hep1 (A) and SNU-449 (B) HCC cells were transiently transfected with indicated siRNA for 96 hours. Expression of YAP1 and TAZ and phosphorylation of S6K1 and S6 were assessed by Western blotting with indicated antibodies.

(C) SK-Hep1 cells were transfected with SLC38A1 and SLC7A5 cDNA after silencing YAP1 and TAZ with specific siRNAs. Expression and phosphorylation of S6K1 and S6 were assessed in cell lysates by Western blotting with indicated antibodies.

(D) Expression of Mst1, Mst2, S6K1, and S6 and phosphorylation of S6K1 and S6 were assessed in liver tissues from wild-type (Wt) and Mst1/2−/− mice (4 weeks old) by Western blotting with indicated antibodies.