Fig 7. Combination of RanGAP1 knockdown by miR-1301 and IM treatment induced nuclear entrapment of BCR-ABL and increased P73 phosphorylation levels in K562 cells.

K562 cells were transfected with pCDH (vector only) or the miR-1301 plasmid and subsequently treated with 250 nM IM for 48 h. The RanGAP1 (A, red) and BCR-ABL protein levels (B, green) and P73 phosphorylation state (Tyr-99) (C, green) were observed using immunofluorescence staining through deconvolution microscopy as described in materials and methods. (D) The quantitative data indicate the nuclear BCR-ABL level in K562 cells (cell number = 15) from (B). The results are presented as means + SD. *p < 0.05, compared with the pCDH (vector only) receiving IM treatment. Arrows denote the position of indicated proteins in K562 cells. Scale bars = 25 μm (A, B). Scale bars = 75 μm (C).