Fig 4. SEAM experimental workflow options.

a) Cells are seeded directly on the culture region of the desired insert at a known density using the seeding module. b) The culture insert with attached cells is transferred to the culture platform for long-term perfusion culture and exposure studies. c) The insert can then be directly transferred for off chip lysis and nucleic acid isolation or d) mounted on a slide for imaging. Cells can also be imaged within the platform using a long working distance objective. e) The modular approach enables a combination of imaging and gene level responses to be easily explored.