Fig 5. SEAM readouts after LPS stimulation.

a) To explore SEAM readouts that mimic vascular inflammation, human microvasular endothelial cells were seeded and transferred to the coupled culture and micro-perfusion modules. b) After apical simulation with LPS for 4 hours, cells were and stained in-channel and the membrane was transferred to a glass slide to explore ICAM1 surface expression after exposure to stimulation. Scale bar = 40 μm. c) To demonstrate rapid and robust RNA isolation, inserts were removed from the architecture and transferred to off-chip RNA isolation. Statistically significant (p<0.001) differences in mRNA expression were observed in ICAM1, CXCL1, CXCL2, and CCL2 with VFW (endothelial marker) remaining constant. SEAM is able to replicate both gene expression and differential surface protein responses to LPS stimulation with an easy to use workflow.