Fig 6. PA21A050 and KAE609 induced Cholesterol incorporation into the parasite is rapidly reversible.

After 2 h of treatment, removal of PA21A050 (A) and KAE609 (B) rapidly leads to restoration of insensitivity to saponin-mediated aldolase loss within 30–60 min. Western blots were probed with antibodies to aldolase and Exp2. (C) After the treatment with PA21A050 (10 nM) or KAE609 (10 nM) for 2 h followed by the removal of compounds, ³H-hypoxanthine incorporation over a 24 h was assessed as a measure of parasite viability. (D) Parasite growth over a 96 h period following the 2 h compound treatment was assessed by measuring parasitemia in Giemsa stained smears every 24 h.