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. 2016 May 26;11(5):e0156239. doi: 10.1371/journal.pone.0156239

Fig 2. Δlam2 cells, Δgtr1 cells and Δgtr2 cells show growth defects in a TORC1-dependent manner.

Fig 2

(A) Growth defects of Δlam2 cells, Δgtr1 cells and Δgtr2 cells were rescued by rapamycin or Torin1. Prototrophic wild-type cells (wt, KP5080), Δlam2 cells (KP6578), Δgtr1 cells (KP6573) and Δgtr2 cells (KP6571) were spotted onto EMM, YES or YPD plates containing 0.2 μg/ml rapamycin or 2 μM Torin1 and incubated as described in Fig 1A. (B) Growth defects of Δlam2 cells, Δgtr1 cells and Δgtr2 cells were rescued by tor2-287 mutation. Prototrophic wild-type cells (wt, KP5080), tor2-287 cells (KP6214), Δlam2 cells (KP6578), tor2-287Δlam2 cells (KP6587), Δgtr2 cells (KP6571), tor2-287Δgtr2 cells (KP6583), Δgtr1 cells (KP6573) and tor2-287Δgtr1 cells (KP6672) were spotted and incubated as described in Fig 1A.