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. 2016 May 26;11(5):e0156456. doi: 10.1371/journal.pone.0156456

Fig 5. T108P reduces trafficking to the cell membrane.

Fig 5

Confocal microscopy of GFP-tagged WT and mutant TASK-2 channels. (A) WT TASK-2 and T108P tagged with GFP at the C-termini expressed in oocytes. The red fluorescent signal (Wheat Germ Agglutinin CF633) indicates the location of the cell membrane. WT channels tagged with GFP (green) exhibit a clear membrane-associated fluorescence, whereas the mutant T108P channels showed no membrane localization, and no GFP fluorescence in any other part of the oocyte. (B) Representative relative signal-intensity profiles for oocytes expressing WT or T108P mutant channels. Intensities were determined along the cross-sections indicated by the red lines in panel A.