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. 2016 May 26;11(5):e0156516. doi: 10.1371/journal.pone.0156516

Fig 4. Growth rate and expression levels in ABCG1- and ABCG4-transfected cells.

Fig 4

(A) HEK293 cells were transiently transfected with ABCG4 (G4), ABCG1 (G1), ABCG2 (G2), or with catalytic site mutant variants of ABCG4 and ABCG1 (G4KM, G1KM). The cell growth of these cultures was monitored for 72 hours. Cells expressing the wild type ABCG4 or ABCG1 protein showed inhibited growth capacity as compared to cells expressing the inactive mutants or the wild type ABCG2 (G2). (B) The expression level of ABCG proteins was also assessed by Western analysis. The total expression of the transgene rapidly declined in the cultures transfected with the wild type ABCG4 or ABCG1, whereas stable expression levels were observed in cells expressing the inactive mutants or the wild type ABCG2. Numbers at the top indicate the elapsed time in hours. (C) Following a three week long selection with G418, no ABCG4 expression was detected in cultures transfected with wild type protein (G4), whereas expression of the inactive mutant (G4KM) persisted. For loading control Na+K+ ATPase was used.