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. 2016 May 26;11(5):e0155780. doi: 10.1371/journal.pone.0155780

Fig 4. Relative expression levels of PsMPT promoter in response to hormone and various abiotic stresses treatments in transgenic Arabidopsis plants.

Fig 4

(A) GUS expressions when exposed to 4°C temperature. Plants were transferred to a cold chamber maintained at 4°C, and the control grew at 18°C. Error bars represent ±SD. (B) GUS staining of transgenic Arabidopsis grew at 18°C (left) and at 4°C for 6 h (right). (C) GUS expressions were measured by qPCR using 35-day-old plants from seeding. 50 μmol L-1 GA3, 100 μmol L-1 NAA, 100 μmol L-1 ABA, 250 mmol L-1 ACC, 200 mmol L-1 NaCl, 40 μmol L-1 mannitol and 100 mmol L-1 PEG was sprayed to the inflorescence at 18°C, and double-distilled water treatment was used as control. (D) GUS fluorescence (nmol L-1 min-1 μg protein-1) were measured by a Microplate Spectrofluorometer using 35-day-old plants from seeding. 50 μmol L-1 GA3, 100 μmol L-1 NAA, 100 μmol L-1 ABA, 250 mmol L-1 ACC, 200 mmol L-1 NaCl, 40 μmol L-1 mannitol and 100 mmol L-1 PEG was sprayed to the inflorescence at 18°C, and double-distilled water treatment was used as control.