Table 1. PCR primers used in this study.
| Target Gene | Primer | Sequence (5’-3’) | Traditional PCR annealing temp. (°C) | qPCR annealing temp. (°C) | Ampliconsize (bp) | Source |
|---|---|---|---|---|---|---|
| blaKPC-2 | K-FW | ATGTCACTGTATCGCCGTCT | 55 | - | 893 | [33] |
| K-RV | TTTTCAGAGCCTTACTGCCC | |||||
| S-blaKPC-2 | SK-FW | GCTTCCCACTGTGCAGCTCATTC | 66.1 | 66.1 | 213 | This study |
| SK-RV | CGCCCAACTCCTTCAGCAACAAATTG | |||||
| S-blaGES-1 | SG-FW | ATGGCACGTACTGTGGCTAA | 56 | 56 | 287 | This study |
| SG-RV | TGACCGACAGAGGCAACTAAT | |||||
| blaIMP-1 | I-FW | GGAATAGAGTGGCTTAAYTCTC | 50 | 50 | 232 | [34] |
| I-RV | GGTTTAAYAAAACAACCACC | |||||
| blaVIM-2 | V-FW | GTTTGGTCGCATATCGCAAC | 60 | - | 382 | [34] |
| V-RV | AATGCGCAGCACCAGGATAG | |||||
| blaOXA-48 | O-FW | GCGTGGTTAAGGATGAACAC | 47 | 47 | 438 | [34] |
| O-RV | CATCAAGTTCAACCCAACCG | |||||
| 16S rRNA | 16S-FW | CGGTGAATACGTTCYCGG | 58 | 57.5 | 126 | [35] |
| 16S-RV | GGWTACCTTGTTACGACTT | |||||
| 16S rRNA | 27F | AGAGTTTGATCCTGGCTCAG | 56 | - | 1466 | [36] |
| 1492R | GGTTACCTTGTTACGACTT |