Table 1. Oligonucleotides used for RT-PCR of Entamoeba histolytica cyst wall proteins.

Sequence Accession	Protein name	Primers F/R 5’ -3’	Amplified size	Annealing T ° C
XM_001913371.1	Malic enzyme, putative	ACTCGGATCCATGGCACAATTAAAAGCAGATTCCTCGAGTTTTCCAGTGACTTTGTTAA	1464	52
XM_645281.2	fructose-1,6-bisphosphate aldolase, putative	ACTCGGATCCATGGCTGCTAAGACTGTTAACATTCCTCGAGATACCATGATTTTCCTGCTGAG	990	61
XM_645264.2	glyceraldehyde-3-phosphate dehydrogenase, putative	ACTCGGATCCATGTCAATTAAGGTCGGTATTAATTCCTCGAGATGAACTTTAGAAATGATTTGGA	1000	58
XM_001914290.1	Gal/GalNAc (Fragment LC3)	TAGAAAGCTTATGTTCTAGTTTAACATGTCCATTTTTCTAGATTAACATGTTTTCTTTGTGTAAATAG	1028	59
XM_001914339.1	Peroxiredoxin	ACTCGGATCCATGTCTTGCAATCAACAAAAAGATTCCTCGAGTCTTGTTTGTTTTAATGTTGTTAA	711	58
AF401986	Chitinase Jessie 1	AAGTGGATCCATGACACTAATTATTTTCTTACCAAGCTTTTATTTATAATTTTCATAAA	273	63
AF401987	Chitinase Jessie 2	AAGTGGATCCATGATGATATTAATCATTTTACCAAGCTTTTAACAATAATCATCATTT	294	65
AF401988	Chitinase Jessie 3	AAGTGAATTCATGAAATTAGCTGTTTTAATACCAAGCTTTTATTTTGAATAATGAACT	1800	65
AF401984.1	Lectin Jacob	AAGTGGATCCATGAAAGCATTACTTGTTATCCCAAGCTTTTAATAACATGGATTGTTA	456	66
XM_652120.2	Chitin Synthase 2,putative	AAGTGGATCCATGTCAGTGAGTTTCCTTATCCCAAGCTTTTACTCTGAATGAGAAAGG	2841	68
AF082517	ADP-ribosylation factor (ARF)	GTAGGACTTGATGCGTGAAGAATTAATGA	259	51