Table 3. Proteins identified as unique with highest peptide-hit score in trophozoites, CLS, and cysts.
| Accession number | Protein name | No. of matched peptides/ Count peptides | Function |
|---|---|---|---|
| Trophozoites | |||
| C4LV11 | Putative uncharacterized protein | 17/35 | unknown function |
| C4LWJ6 | LIM zinc finger domain containing protein | 9/32 | zinc ion binding |
| C4LV07 | Malate dehydrogenase, putative | 7/30 | oxidoreductase activity |
| C4LT49 | 40S ribosomal protein S24, putative | 11/29 | translation |
| B1N3R9 | 60S ribosomal protein L7a, putative | 10/27 | ribosome biogenesis |
| C4M0A8 | Putative uncharacterized protein | 16/26 | unknown function |
| C4M0Q2 | 40S ribosomal protein S8 | 12/24 | translation |
| C4M727 | 60S ribosomal protein L27, putative | 11/24 | translation |
| C4LUC7 | Non-conventional myosin IB | 11/23 | ATP binding |
| C4LW30 | RNA modification enzymes, MiaB-family | 12/23 | RNA modification |
| C4LZB0 | 40S ribosomal protein S6 | 8/23 | translation |
| C4M7H1 | Actobindin, putative | 10/23 | binds actin monomers |
| C4LZ12 | Tyrosyl-tRNA synthetase, putative | 9/22 | tRNA aminoacylation for protein translation |
| B1N2Z3 | 60S acidic ribosomal protein P0, putative | 10/21 | ribosome biogenesis |
| C4LSV0 | ARP2/3 complex 20 kDa subunit, putative | 10/21 | actin filament polymerization |
| CLS | |||
| B1N4A1 | Grainin, putative | 9/22 | calcium ion binding |
| C4M784 | Putative uncharacterized protein | 10/18 | catalytic activity |
| C4LTM6 | Serine-threonine-isoleucine rich protein, putative | 8/15 | integral component of membrane |
| C4LU65 | Vacuolar ATP synthase subunit H, putative | 6/14 | ATP hydrolysis coupled proton transport |
| B1N330 | Serine-threonine-isoleucine rich protein, putative | 6/11 | integral component of membrane |
| C4LU31 | Thioredoxin, putative | 4/8 | cell redox homeostasis |
| C4LUQ2 | Glucosidase, putative | 3/6 | carbohydrate metabolic process |
| C4LTT0 | Cysteine proteinase, putative | 3/6 | cysteine-type peptidase activity |
| C4LTV9 | Uncharacterized protein | 3/5 | unknown function |
| C4LV34 | Putative uncharacterized protein | 2/5 | unknown function |
| C4LT65 | Heat shock protein 70, putative | 1/4 | Stress response |
| C4M916 | Copine, putative | 2/4 | calcium-dependent membrane binding proteins |
| C4LX23 | RhoGAP domain containing protein | 2/4 | signal transduction |
| C4M6M0 | Putative uncharacterized protein | 1/4 | small GTPase mediated signal transduction |
| C4M741 | Cortexillin, putative | 2/4 | Cytokinesis |
| Cyst | |||
| C4M4Q8 | Nuclear transcription factor, putative | 1/29 | sequence-specific DNA binding |
| C4M6B9 | Putative uncharacterized protein | 1/23 | RNA binding |
| B1N624 | Aminoglycoside 3'-phosphotransferase, putative | 4/16 | response to antibiotic |
| C4M8Z0 | Alkyl sulfatase, putative | 1/15 | alkyl sulphatase activity |
| C4MBE9 | Putative uncharacterized protein | 2/12 | unknown function |
| C4M1F3 | Putative uncharacterized protein | 1/11 | unknown function |
| C4M977 | Putative uncharacterized protein | 1/11 | rRNA processing |
| C4LW71 | DNA mismatch repair protein PMS1, putative | 1/7 | mismatch repair |
| C4LX70 | Putative uncharacterized protein | 1/5 | double-strand break repair via non-homologous |
| C4M4W5 | Putative uncharacterized protein | 1/5 | unknown function |
| C4LYB9 | Putative uncharacterized protein | 1/4 | unknown function |
| C4M2G7 | Putative uncharacterized protein | 1/4 | phosphoric ester hydrolase activity |
| B1N3D1 | Tyrosine-protein kinase 2, putative | 1/3 | ATP binding |
| B9U2P3 | Organellar DNA polymerase 1 | 2/3 | DNA replication |
| C4LV85 | Centromeric protein E, putative | 2/3 | kinesin-like motor protein |
Molecular function shown in italics. Biological function shown in bold. Unknown function: Unknown function in E. histolytica.