Table 2. Primer sequences of the virulence related genes and EF1α (used as a reference gene) used for qRT-PCR.

Target gene	Forward primer sequence (5’ to 3’)	Reverse primer sequence (5’ to 3’)	Amplicon size
Chitin synthase	GGCACAAGGATGAACAACTGGGA	GGAGCTGGATTGAGCATAGGGATC	99 bp
Cell wall protein	GGCAAGCCTTACACCATCCGCTAC	TGTAGTCAGAGAGATCATCGGAGG	91 bp
Mitochondrial carrier protein	CCGCGTTGAGATGCAGAGCAAGAA	GACGCCGTTGGTCTCGTAAATGTAC	97 bp
Glucanosyltransferase	GGCTACATCTGCGGCCAAGACAAG	GAGCACATGCTGTAGGCACCATAG	89 bp
G protein β subunit	GGTCGACCGATAGGAGGCACC	GTGGACCTTGTTGGTGGTATAGGC	86 bp
Xylanase	CCGGCGACGATGTGATGCGA	CCCAGGTGTGGTTGCTCGCT	82 bp
Pectate lyase	GCGGTGCTTTCCATGCTAGCG	GACGAGCTTTCCGTAGTCTTCGGC	98 bp
Polygalacturonase	CTCGCCACTCGACTTGACCTGG	TGAAGCTGTGGTCTGCCCAGTAG	101 bp
EF1α	AGCTCGGTAAGGGTTCCTTC	TCCAGAGAGCAATATCGATGG	93 bp