Table 3. Phenotypic characterization of eGFP transformants of Foc 2.
| F. oxysporum f.sp. ciceri race 2 Wild type and Transformant | Mycelial growth | RFU/μg of protein | |
|---|---|---|---|
| RMGF (mm) | RGR (mm/h) | ||
| Wild type | 20.2 | 0.15 | 2.11±0.06 |
| A1 | 18.9 | 0.13 | 40.06±0.01 |
| A3 | 19.3 | 0.13 | 18.94±0.01 |
| C2 | 19.4 | 0.13 | 20.12±0.097 |
| C4 | 19.1 | 0.13 | 49.45±0.14 |
| D4 | 18.7 | 0.13 | 60.84±0.17 |
A1, A3, C2, C4 and D4 are the isolates transformed with the eGFP gene
RMGF: Radial mycelial growth final, assessed by the average value of the fungal colony radius reached after 9 days growth at 25°C under light.
RGR: Radial growth rate; calculated by slope of linear regression of the mean colony radius over time. Each value is the mean of three replicates (petri dishes).
RFU: Relative fluorescence units.