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. 2016 May 26;11(5):e0156496. doi: 10.1371/journal.pone.0156496

Fig 2. USP18 expression is efficiently suppressed in lentiviral-vectors-transduced HepG2.2.15 cells.

Fig 2

(A) Analysis of USP18 mRNA levels. Hepg2.2.15 cells were treated with ShUSP18-1, -2, -3, -4 and shRR. Quantitative real-time PCR analysis was performed to analyze the level of USP18 mRNA 72 hours after transduction. (B) Fluorescence images of Hepg2.2.15 cells transfected for 72 hours in six-well plates with shUSP18–1 per well (MOI = 10). GFP expression was observed under light (a) and fluorescence (b) microscopy (×100). (C) Protein was collected after transduction of shUSP18-1 and levels of USP18 and ISG15 protein in each group were determined by western blot. Lane 1: shUSP18–1 lentivirus group. Lane 2: shRR lentivirus group. Lane 3: normal control group. The results are presented as the means ± SD, n = 3, error bars indicate SD. *P < 0.05; **P < 0.01 vs. control.