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. 2016 May 26;11(5):e0156496. doi: 10.1371/journal.pone.0156496

Fig 4. USP18 silencing enhances the antiviral activity of IFN-α against HBV expression in Hepg2.2.15 cells.

Fig 4

(A) After treated with indicated dose of IFN-α (0, 1, 10, 100, 1000 IU/ml) in USP18 silenced Hepg2.2.15 cells, the supernatant was collected for detection of HBV DNA load using quantitative real-time PCR kits. (B) and (C) The levels of HBsAg and HBeAg were detected using ELISA kits. The secretion of HBV DNA, HBsAg and HBeAg was significantly inhibited by shUSP18. (D) The level of intracellular HBcAg was quantified by western blot. The results are presented as the means ± SD, n = 3, error bars indicate SD. *P < 0.05; **P < 0.01 vs. control.