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. 2016 May 26;11(5):e0156451. doi: 10.1371/journal.pone.0156451

Fig 6. E-cigarettes can Induce α7 Expression at the Transcriptional, Translational, and Protein Levels.

Fig 6

(A) Transient transfection showed that 1.5μM of three different brands of e-cigarettes could induce the α7 promoter after 48 hours of stimulation in A549 cells, to a similar extent as nicotine. (B and C) Immunofluorescent staining and western blot analysis showed that 1.5μM of e-cigarettes could induce α7 at the protein level after 48 hours in A549 cells, to a similar extent as nicotine. For western blot, arrow indicates the α7 protein band and quantification of the data is depicted in the corresponding graphs. (D) qRT-PCR analysis showed that 1.5μM of e-cigarettes resulted in increased α7 mRNA levels after 48 hours, decreased α3 mRNA levels after 24 hours, and increased α5 mRNA levels after 48 hours.