Table 3.
Order-of-addition RNase H inhibition assay results in the presence of excess RT
| % Inhibition | ||
|---|---|---|
| 1. Pre-Incubation (10 min at 37 °C) |
RT + Inhibitor | RT + HTS-1 |
| 2. Reaction Intiation (2 min at 37 °C) |
HTS-1 + MgCl2 |
Inhibitor + MgCl2 |
| 10qa | 70% ± 9%b | 58% ± 3% |
| 10r | 82% ± 1% | 61% ± 8% |
| 10t | 42% ± 1% | 15% ± 5% |
| 10v | 78% ± 2% | 59% ± 6% |
| 10x | 91% ± 0% | 74% ± 4% |
| 10y | 92% ± 1% | 80% ± 1% |
| β-thujaplicinol | 100% ± 0% | 98% ± 4% |
a
Final reaction conditions: 300 nM HIV RT, 50 nM HTS-1, 0.5 mM EDTA, 6 mM MgCl2, 30 µM inhibitor.
b
Values are reported as mean ± standard deviation from two independent experiments.