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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Mar 24;36(6):1197–1208. doi: 10.1161/ATVBAHA.116.307421

Figure 5. LPA/PKD-1-CD36 signaling axis regulates the expression of angiogenic and arteriogenic genes.

Figure 5

A, Overexpression of PKD-CA increases the expression of EFNB2 mRNA level. HMVEC-C (passage 2) transduced with GFP or PKD-1:GFP were processed for total RNA and mRNA expression by quantitative real time RT-PCR. B, Clone 3 TAEC cells were processed as in Figure 4A and cell lysates collected and subjected to Western blots with anti-ephrinB2 antibody. Shown is a representative result from duplicate experiments. C, The impact of PKD-CA overexpression on angiogenic gene expression. HMVEC-C transduced with GFP or PKD-1:GFP were processed for total RNA and mRNA expression of NRP1, DLL4 and HEY2 by quantitative real time RT-PCR. D, Overexpression of human CD36 downregulates EFNB2 expression in HMVEC-C. HMVEC-C (passage 2) transduced with GFP or hCD36:GFP were processed for total RNA and mRNA expression by quantitative real time RT-PCR. E, Overexpression of human CD36 does not impact the expression of NRP1 (neuropilin1), DLL4 (delta like ligand 4) and HEY2 in HMVEC-C. HMVEC-C (passage 2) was processed as described in D for quantitative real time RT-PCR.