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Preparation of t-butyl ester analogues of 5 and the assessment of the ester analogues of 5 (above) and 6 (Scheme 4) assayed (50 µM) against mouse macrophages alongside LPS (1 ng/mL) for stimulated release of TNF-α. All data is the mean of triplicate assay versus vehicle control, error bars represent SEM. * = P ≤ 0.05; ** = P ≤ 0.01; *** = P ≤ 0.001.
