Table 1.
List of primary antibodies used in this study.
Antibody | Antigen | Host, type | Dilution | Source (Cat. No.) |
---|---|---|---|---|
Calbindin D-28K | Recombinant rat calbindin D-28K | Rabbit, polyclonal | 1:500 | Swant, Marly, Switzerland (CB-38) |
Calretinin | Guinea pig calretinin, full length amino acid sequence | Goat, polyclonal | 1:500 | Merck-Millipore, Darmstadt, Germany (AB 1550) |
ChAT | Human placental choline acetyltransferase | Goat, polyclonal | 1:250 | Merck-Millipore (AB 144P) |
Cx30.2 | Peptide derived from amino acids 92–109 of mouse Cx30.2 | Guinea pig, polyclonal | 1:500 | Pineda Antibody Service, Berlin, Germany |
Cx30.2 | Peptide derived from the last 20 amino acids of the C-terminal end of mouse Cx30.2 | Rabbit, polyclonal | 1:5000 (wb) 1:500 (He) | Pineda Antibody Service |
Cx36 | C-terminal region of rat and mouse Cx36, derived from amino acids 286–303 | Mouse, monoclonal | 1:500 | Invitrogen (37-4600) |
Disabled-1 | GST-mDisabled-1 fusion protein, corresponding to amino acids 107–243 | Rabbit, polyclonal | 1:500 | Gift from Dr B. Howell, NIH |
β-Galactosidase | Full length purified β-gal from Escherichia coli | Chicken, polyclonal | 1:500 | Abcam, Cambridge, MA, USA (ab9361) |
GFP | Full-length Aequorea victoria GFP | Mouse, monoclonal | 1:5000 | Clontech, Mountain View, CA, USA (632380) |
Melanopsin | 15 most N-terminal amino acids of the mouse melanopsin extracellular domain | Rabbit, polyclonal | 1:5000 | Advanced Targeting Systems, San Diego, CA, USA (AB-N38, UF006) |
Parvalbumin | Rat muscle parvalbumin | Rabbit, polyclonal | 1:5000 | Swant (PV28) |
Dilutions were identical for labeling of cryosections, retinal whole-mounts, Western blot (wb) and HeLa cell experiments (He) if not stated otherwise.