TABLE 1.
The 25 proteins with the highest synthesis rate in allicin-treated E. coli identified from two-dimensional gels (see Fig. 5)
NA, not applicable.
| Spot ID | Protein (EG number) (53) | Protein function (53) | Theoretical molecular mass | Theoretical pI (pH) |
|---|---|---|---|---|
| Da | ||||
| 1 | ALI1 (NA) | Unidentified allicin-induced protein 1 | NA | NA |
| 2 | RplL (EG10873) | 50S ribosomal subunit protein L7/L12 | 12,287 | 4.3989 |
| 3 | GroS (EG10600) | Chaperonin Cpn10 | 10,380 | 4.9585 |
| IbpB (EG11535) | Chaperone, heat-inducible protein of HSP20 family | 16,083 | 5.02 | |
| 4 | AhpC (EG11384) | Alkyl hydroperoxide reductase, subunit C | 20,748 | 4.8486 |
| 5 | ALI5 (NA) | Unidentified allicin-induced protein 5 | NA | NA |
| 6 | RbsD (EG10817) | d-Ribose pyranase | 15,282 | 5.9326 |
| RplS (EG10880) | 50S ribosomal subunit protein L19 | 13,125 | 11.0625 | |
| 7 | ALI7 (NA) | Unidentified allicin-induced protein 7 | NA | NA |
| 8 | AhpC (EG11384) | Alkyl hydroperoxide reductase, subunit C | 20,748 | 4.8486 |
| 9 | FliC (EG10321) | Flagellin, structural gene | 51,264 | 4.3008 |
| 10 | RplL (EG10873) | 50S ribosomal subunit protein L7/L12 | 12,287 | 4.3989 |
| 11 | TrxC (EG11887) | Thioredoxin 2, zinc binding | 15,544 | 4.8164 |
| 12 | NfuA (EG12935) | Fe/S biogenesis protein | 20,984 | 4.3271 |
| 13 | Hns (EG10457) | DNA-binding global regulator H-NS | 15,530 | 5.2412 |
| 14 | IbpA (EG11534) | Chaperone, heat-inducible protein of HSP20 family | 15,764 | 5.4785 |
| 15 | Lpp (EG10544) | Murein lipoprotein | 8,318 | 9.7734 |
| YbeD (EG11592) | UPF0250 family protein | 9,821 | 5.4053 | |
| 16 | GrpE (EG10416) | Nucleotide exchange factor for the DnaKJ chaperone | 21,784 | 4.4839 |
| 17 | RpsM (EG10912) | 30S ribosomal subunit protein S13 | 13,091 | 11.2163 |
| IbpB (EG11535) | Chaperone, heat-inducible protein of HSP20 family | 16,083 | 5.02 | |
| UspA (EG11390) | Global regulatory gene for stress response | 16,056 | 4.9219 | |
| 18 | GroS (EG10600) | Chaperonin Cpn10 | 10,380 | 4.9585 |
| IbpB (EG11535) | Chaperone, heat-inducible protein of HSP20 family | 16,083 | 5.02 | |
| 19 | ZapB (EG11878) | FtsZ stabilizer, coiled coil protein | 9,628 | 4.4883 |
| 20 | ALI20 (NA) | Unidentified allicin-induced protein 20 | NA | NA |
| 21 | ZapB (EG11878) | FtsZ stabilizer, coiled coil protein | 9,628 | 4.4883 |
| 22 | FliC (EG10321) | Flagellin, structural gene | 51,264 | 4.3008 |
| 23 | TrxA (EG11031) | Thioredoxin 1 | 11,799 | 4.4751 |
| 24 | NfuA (EG12935) | Fe/S biogenesis protein | 20,984 | 4.3271 |
| 25 | OmpA (EG10669) | Outer membrane protein A | 37,177 | 5.9678 |