FIGURE 7.
Emodin inhibits LPS/IFNγ- and IL4-induced histone modifications in macrophages. Macrophages were stimulated with LPS (100 ng/ml) and IFNγ (20 ng/ml) or IL4 (10 ng/ml) with or without emodin (50 μm) for 24 h. A, global histone modification levels were detected using Western blotting. The experiment was performed in triplicate. Con, control; Em, emodin. B and C, ChIP-PCR was used to detect gene-specific changes in histone modifications. Results are shown as the mean ± S.E. (n = 3). *, p ≤ 0.05; **, p ≤ 0.01.