FIGURE 10.
Macrophages in the PtenΔhep mice are highly susceptible to LPS. A, hepatic macrophages isolated from the Ptenfl/fl and the PtenΔhep mice were stimulated with LPS at the indicated concentration. The concentrations of IL-6 and TNFα in the supernatant are presented (n = 4 each). B, peritoneal macrophages isolated from the Ptenfl/fl and the PtenΔhep mice were stimulated with LPS at the indicated concentration. The concentrations of IL-6 and TNFα in the supernatant are presented (n = 5 each). C, mice primed with P. acnes were injected with LPS (n = 10 each). The serum IL-6, TNFα, and GPT levels are presented as the indicated time points. D, hydrogen peroxidase content in non-tumor liver tissue (n = 5 each, 48 weeks of age). E, serum GPT levels in the Ptenfl/fl and the PtenΔhep mice stimulated with LPS after treated with or without the anti-oxidant N-acetyl-l-cysteine. Serum samples were collected at 6 h after the LPS injection (n = 4 each). Differences between two groups were compared using the Mann-Whitney U test. The data are presented as the mean ± S.E. *, p < 0.05. NS, not significant.