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Isolation of EpCAM-positive Huh 7 cells. Huh 7 cells were divided into EpCAM-positive cells and EpCAM-negative 7 cells using magnetically activated cell sorting. A, EpCAM mRNA expression examined by quantitative real time PCR (n = 4 each). Differences between the two groups were compared using the Mann-Whitney U test. The data are presented as the mean ± S.E. *, p < 0.05. B, EpCAM-positive cells were successfully isolated from Huh 7 cells.
