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AKT activation in primary cultured hepatocytes. Hepatocytes were isolated from the Ptenfl/fl mice (fl/fl), the PtenΔhep mice (Δhep), and the PtenΔhep/T4−/− mice (Δhep/T4−/−) at 12 weeks of age. These primary-cultured hepatocytes were stimulated with or without 10 μg/ml insulin and harvested at 20 min after stimulation. Western blotting for p-AKT (Ser-473), AKT, and actin are shown.
