Macrophage M1 and M2 expression in isolated macrophages. Hepatic macrophages were isolated from the Ptenfl/fl mice (f/f), the PtenΔhep mice (Δhep), and the PtenΔhep/T4−/− mice (Δhep/T4−/−) (n = 4 each). M1 markers (A) and M2 markers (B) were examined by quantitative real time PCR. Differences between two groups were compared using the Mann-Whitney U test. The data are presented as the mean ± S.E. *, p < 0.05, compared with Ptenfl/fl mice. #, p < 0.05, compared with PtenΔhep mice.