TABLE 1.

List of primary antibodies

The following abbreviations are used: WB, Western blotting; IHC, immunohistochemistry; IF, immunofluorescent staining; AFP, α-fetoprotein.

Antibody	Dilution	Catalog no.	Distributor
Actin	WB (×10,000)	A4700	Sigma
AFP	IHC (×50)	sc-8108	Santa Cruz Biotechnology
AKT	WB (×1000)	9272S	Cell Signaling
p-AKT (Ser-473)	WB (×1000)	9271	Cell Signaling
EpCAM	WB (×1000)	Ab71916	Abcam
	IHC (×200)
F4/80	IHC (×100)	14-4801	eBioscience
Ki67	IHC (×400)	12202	Cell Signaling
Ly6C	IHC (×100)	Ab15627	Abcam
	IF (×50)
PCNA	IHC (×100)	M0879	Dako