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Activation of mycobacterial P1B4-ATPases by Fe2+. ATPase activity of purified MsCtpJ (A), MtCtpD (B), and MtCtpJ (C) in the presence of a 0.1 (green bars) or 1.0 mm (blue bars) concentration of the indicated metal ions was determined. D, Fe2+ dependence of MsCtpJ (■; blue), MtCtpD (●; black), and MtCtpJ (♦; green) ATPase activities. Data are the mean ± S.E. (error bars) of three independent experiments performed in duplicate.
