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Yeast viability is dependent on CTD length. A, schematic depicting RPB1 mutants with variable length CTDs. Each seven-amino acid (21 base pairs) repeat is shown as a single green box. B, strains expressing CTD mutants with as few as ten repeats show near wild-type growth; however, a mutant with only eight repeats is severely compromised as reported by others using the classic URA3 shuffling system. Spotting assays are representative examples of at least three independent trials.
