TABLE 2.
Plasmids used in this work
| Plasmid | Reference | Notes |
|---|---|---|
| pL-RPB1 | Ref. 9 | |
| pRS315 | Ref. 14 | Referred to as pLEU in the text |
| pVS31 | Ref. 11 | Plasmid for making pif1-m2 mutation |
| pJMD2 | Ref. 13 | Plasmid for recursive directional ligation used to make CTD mutants |
| pSMF2 | This work | pL-RPB1 with XmaI and SacI sites flanking the CTD coding region. Referred to as pRPB1 in the text |
| pRPB1-CTD8 | This work | pSMF2 with 8 CTD repeats |
| pRPB1-CTD10 | This work | pSMF2 with 10 CTD repeats |
| pRPB1-CTD14 | This work | pSMF2 with 14 CTD repeats |
| pRPB1-CTD26 | This work | pSMF2 with 26 CTD repeats |
| pRPB1–4stop | This work | pSMF2 with repeats 8–10 interrupted with 4 repeats containing stop codons |