TABLE 3.

Analysis of CTD rearrangements

The rates of suppressor formation were determined by measuring the frequencies of fast growing colonies on SC−Leu plates containing doxycycline. Rates and 95% confidence intervals (in parentheses) were calculated using the MSS-maximum likelihood estimator method (16, 17).

Reporter	Rate × 10−6/cell/generation	Total	Expansion	Contraction	Recombination	Other	Expansion	Contraction
							%	%
pRPB1–4stop	26 (19–42)	115	1	59	NDa	55	<1	51
pRPB1-CTD8	4.5 (2.5–6.9)	169	65	0	15	89	38	0

^a Homologous recombination events cannot be distinguished from extragenic suppressors by colony PCR so they are included into the “other” category.