TABLE 1.
Candidate proteins for αvβ8 integrin ligands
| Gene symbols | Protein names | N-terminal tag | C-terminal tag | Purification methods |
|---|---|---|---|---|
| ANGPTL7 | Angiopoietin-related protein 7 | FLAG | His | Ni-NTA and anti-FLAG |
| BIGH3 | Transforming growth factor-β induced protein ig-h3 | FLAG | His | Ni-NTA and anti-FLAG |
| ECM2a | Extracellular matrix protein 2 | FLAG | His | Ni-NTA and anti-FLAG |
| EDIL3 | EGF-like repeat and discoidin l-like domain containing protein 3 | FLAG | His | Ni-NTA and anti-FLAG |
| EGFL6 | EGF-like protein 6 | GST | 38 | |
| EGFLAM | EGF-like, fibronectin type III, and laminin G domains (pikachurin) | FLAG | His | Ni-NTA and anti-FLAG |
| F2 | Prothrombin | FLAG | His | Ni-NTA and anti-FLAG |
| FBLN5 | Fibulin-5 | FLAG | His | Ni-NTA and anti-FLAG |
| FBN1b | Fibrillin-1 | FLAG | His | Ni-NTA and anti-FLAG |
| FBN2b | Fibrillin-2 | FLAG | His | Ni-NTA and anti-FLAG |
| FINCc | Fibronectinc | 27 | ||
| FREM1 | FRAS1-related extracellular matrix protein 1 | GST | 37 | |
| HMCN2b | Hemicentin-2 | FLAG | His | Ni-NTA and anti-FLAG |
| IBSP | Bone sialoprotein 2 | FLAG | His | Ni-NTA and anti-FLAG |
| IGFBP2 | Insulin-like growth factor-binding protein 2 | FLAG | His | Ni-NTA and anti-FLAG |
| KARSa | Lysine-tRNA ligase | FLAG | His | Ni-NTA and anti-FLAG |
| LAMA5 | Laminin α5 subunit | 36 | ||
| MFGE8b | Lactadherin | FLAG | His | Ni-NTA and anti-FLAG |
| NPNT | Nephronectin | FLAG | 24 | |
| NTN1 | Netrin-1 | FLAG | His | Ni-NTA and anti-FLAG |
| PCSK5a | Proprotein convertase subtilisin/kexin type 5 | FLAG | His | Ni-NTA and anti-FLAG |
| PCSK6b | Proprotein convertase subtilisin/kexin type 6 | FLAG | His | Ni-NTA |
| SEMA3Ca | Semaphorin-3C | FLAG | His | Ni-NTA and anti-FLAG |
| SPP1 | Osteopontin | FLAG | His | Ni-NTA and anti-FLAG |
| TGFB1c | Transforming growth factor-β1c | His | Ni-NTA | |
| THBS1b | Thrombospondin-1 | FLAG | His | Ni-NTA |
| THBS2 | Thrombospondin-2 | FLAG | His | Ni-NTA and anti-FLAG |
| VTNc | Vitronectinc | FLAG | His | Ni-NTA |
| WNT10A | Wingless-type MMTVd integration site family, member 10A | FLAG | His | Ni-NTA and anti-FLAG |
a The candidate proteins that could not be purified because of low expression are shown.
b These are known αvβ8 ligands.
c The candidate proteins that were purified as truncated forms are shown.
d MMTV is murine mammary tumor virus.