Skip to main content
. 2016 Apr 12;291(22):11689–11697. doi: 10.1074/jbc.M116.719997

FIGURE 4.

FIGURE 4.

FLCN recruits LKB1 for AMPK activation in response to flow stress. A, extracts from HKC-8 cells grown under flow (Flow +) or no-flow (Flow −) condition were immunoprecipitated with anti-FLCN antibody (α-FLCN). The levels of the indicated proteins in the extracts (Ext) and precipitates (IP) were determined by Western blotting. B, HKC-8 cells stably expressing doxycycline inducible LKB1 shRNA were grown under flow or no-flow condition in the presence (Dox +) or absence (Dox −). The expression levels of LKB1, AMPK, actin, and phosphorylation of AMPK were analyzed by Western blotting. C, extracts from HKC-8 cells grown under flow (Flow +) or no-flow (Flow −) condition were immunoprecipitated with anti-LKB1 antibody (α-LKB1) or control IgG. D, HKC-8 cells stably expressing doxycycline inducible FLCN shRNA were grown under flow condition in the presence (Dox +) or absence (Dox −) of doxycycline. Extracts from the treated cells were immunoprecipitated with anti-LKB1 antibody (α-LKB1) or control IgG. E, HKC-8 cells stably expressing doxycycline inducible IFT88 shRNA were grown under flow condition in the presence (Dox +) or absence (Dox −) of doxycycline. Extracts from the treated cells were immunoprecipitated with anti-LKB1 antibody (α-LKB1) or control IgG.