FIGURE 4.

Pol III* reconstituted with DnaX complexes containing τ₃, τ₂γ, and τγ₂ produce the same-length lagging strand products. A, an optimized rolling circle reaction was conducted with Pol III* reconstituted with the designated DnaX complexes. α-[³²P]dCTP or α-[³²P]dGTP labeled the leading and lagging strands, respectively. B, optimized rolling circle reactions were conducted in the presence of either 100 μm dGTP or the indicated amount of dGDPNP. α-[³²P]dATP (2000 cpm/pmol) was used to label both the leading and lagging strands, which could be distinguished by their length after alkaline-agarose gel electrophoresis.