FIGURE 10.
Dissociation of the 95-kDa caveolin4–5 oligomer to monomers and dimers. Shown are 10 μg of ROV samples solubilized at different conditions; Western blotting with anti-caveolin antibody. Lane 1, ROVs with no treatment; lane 2, ROVs solubilized with 5 mg/ml urea and 4 mg/ml DDM incubated for 30 min at room temperature; lane 3, ROVs solubilized with 5 mg/ml urea and 4 mg/ml DDM heated for 30 min at 65 °C; lane 4, ROVs solubilized with 5 mg/ml urea and 4 mg/ml DDM incubated with 10 mm DTT for 15 min at room temperature and then with 20 mm iodoacetamide for 15 min (n = 4).