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. 2016 Apr 4;291(22):11751–11760. doi: 10.1074/jbc.M115.708438

FIGURE 3.

FIGURE 3.

Effect of the B1B2 and B3B4 regions of PEX9 on the inhibition of gelatin degradation by MMP-9. A, schematic drawing of the truncated GST-B1B2 and GST-B3B4 fusion proteins prepared in this study. B, SDS-PAGE analysis of the purified GST fusion proteins, visualized by Coomassie Blue staining. C, representative gelatin zymography analysis using 20 ng of proMMP-9 and with or without the indicated proteins (0.4 μm each). D, 60 nm of MMP-9 was added to FITC-gelatin-coated plates in the absence or presence of 0.4 μm of the indicated proteins. After 24 h at 37 °C, fluorescence was determined, and control values were normalized to 100. E, effect of the indicated proteins on the conversion of DQ-gelatin into fluorogenic gelatin. The lines show the maximal enzyme velocity evolution as a function of the amount of substrate. The bars represent standard deviation. *, p < 0.05; **, p < 0.01. AU, arbitrary units.