TABLE 1.
Primers designed to generate the various GST-PEX9 recombinant proteins
F, forward; R, reverse.
| Protein | Primers (5′ → 3′) |
|---|---|
| PEX9 F | GAATTCCCCTTTGAGTCCGGTGGACG |
| PEX9 R | CTCGAGCTAGTCCTCAGGGCACTGCA |
| B1B2 F | GAATTCCCCTTTGAGTCCGGTGGACG |
| B1B2 R | CTCGAGTCACCTGGGCCACGTC |
| B3B4 F | GAATTCAGCCGACGTGGCCCAG |
| B3B4 R | CTCGAGCTAGTCCTCAGGGCACTGCA |
| B1 F | GAATTCCCCTTTGAGTCCGGTGGACG |
| B1 R | CTCGAGTCCAGCTTGCGGGGCA |
| B2 F | GAATTCACCGCTGGACTCGGTCTTTG |
| B2 R | CTCGAGTCACCTGGGCCACGTC |
| B3 F | GAATTCAGCCGACGTGGCCCAG |
| B3 R | CTCGAGTCCAAAGGCACCCCGG |
| B4 F | GAATTCTTTGGACACGCACGACG |
| B4 R | CTCGAGCTAGTCCTCAGGGCACTGCA |
| ΔB1 F | TCTAGAGCTGGACTCGGTCTTTGA |
| ΔB1 R | TCTAGAGATCCACGCGGAACCAGAT |
| ΔB2 F | TCTAGAAGTGGCAGGGGGAAGATG |
| ΔB2 R | TCTAGAGCCACTTGTCGGCGATA |
| ΔB3 F | TCTAGAGACGTCTTCCAGTACCGAGAG |
| ΔB3 R | TCTAGAGCCCAGCTTGTCCAGA |
| ΔB4 F | TCTAGAGACTGACTGACGATCTGCCTC |
| ΔB4 R | TCTAGATCCGGGGATCCACCAT |