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Apoptotic activities of Bid and its mutants when expressed in Bid KO cells. Bid KO HCT116 clone 1 cells carrying Dox-inducible expression viruses for GFP, Bid, Bid D60E, or Bid G94E were treated with Dox (1 μg/ml) for the indicated time. Cells were harvested at the end of the Dox treatment and assayed for protein expression and PARP cleavage by Western blot.
