TABLE 1.

Expression analysis of denitrification genes using qRT-PCR in wild type and mutants of glyoxylate shunt gene

Name	Locus tag	Protein name	-Fold changea
			ΔaceA/WT	ΔglcB/WT
narH	PA3874	Respiratory nitrate reductase β chain	3.21 ± 0.18	1.37 ± 0.41
napA	PA1174	Periplasmic nitrate reductase protein NapA	2.92 ± 0.41	1.95 ± 0.31
narG	PA3875	Respiratory nitrate reductase α chain	2.37 ± 0.47	1.13 ± 0.42
norD	PA0525	Probable denitrification protein NorD	2.32 ± 0.06	1.49 ± 0.12
norB	PA0524	Nitric-oxide reductase subunit B	2.30 ± 2.23	1.00 ± 0.12
narX	PA3878	Two-component sensor NarX	2.14 ± 0.32	1.99 ± 0.31
nirS	PA0519	Nitrite reductase precursor	2.04 ± 0.21	1.24 ± 0.19
narJ	PA3873	Respiratory nitrate reductase delta chain	1.92 ± 0.31	3.41 ± 0.51
nosZ	PA3392	Nitrous-oxide reductase precursor	1.83 ± 0.12	1.47 ± 0.04
norC	PA0523	Nitric-oxide reductase subunit C	1.77 ± 0.14	1.32 ± 0.31
narI	PA3872	Respiratory nitrate reductase γ chain	1.73 ± 0.41	2.12 ± 0.41
napB	PA1173	Cytochrome c-type protein NapB precursor	1.64 ± 0.47	2.32 ± 0.28

^a The average values with standard deviation were obtained from duplicate biological independent experiments, and qRT-PCR reaction was performed in triplicate. -Fold change represents relative expression to wild-type in exponential phase.