FIGURE 9.

ES-62 added to serum reduces classical complement activation and the rate-limiting factor active C4. A, diagram of the effects of different PCh forms on the complement pathway. B, classical complement activation was measured by C3d deposition onto IgM-coated microtiter plates. Serum diluted 1 to 100 in VBSCaMg was either left untreated or treated with ES-62 (0.1 μg/ml) for 30 min at 37 °C prior to addition to the IgM. Statistical analysis was undertaken by Wilcoxon matched pairs test (n = 14 different sera in three different experiments). C, active C4 was measured following addition of ES-62 (0.5 μg/ml) to serum diluted 1 in 100 and incubation for 45 min at 37 °C (n = 6 different donor sera). D, as for B, but CRP was added at 0.4 μg/ml final concentration. Data for 12 different donor sera in two separate experiments were analyzed by Wilcoxon matched pairs test.