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. 2016 May 27;6:26827. doi: 10.1038/srep26827

Figure 1. Differential replication efficiencies of HEV in different human hepatocyte derived cell lines.

Figure 1

S10-3 (a), Huh7.5 (b) and HepG2/C3A (c) cells were transfected with either wild-type HEV Rluc or HEV Rluc GAA (GDD mutant) RNA (2 μg/well). Cells were co-transfected with firefly luciferase plasmid DNA (pGL-3 promoter vector, 100 ng/well) to normalize cell transfection efficiency and the Renilla luciferase signal. Cell associated Renilla luciferase activity was checked to monitor HEV replication from 1-6 days. The data represents mean ± SD of three independent triplicate set of experiments, [** and ***represent p < 0.01 and p < 0.001 respectively, statistical comparisons were done by one-way analysis of variance (ANOVA) between mock transfected cells, and cells transfected with HEV-Rluc RNA].