Skip to main content
. 2016 May 27;6:26827. doi: 10.1038/srep26827

Figure 2. Interferon-α treatment inhibits HEV replication in Huh7.5 cells.

Figure 2

(a) Huh7.5 cells transfected with HEV Rluc replicon RNA were treated with IFN-α (1,000 U/ml) 4 h post transfection. Cells were assayed for luciferase activity to monitor HEV RNA replication as described in Fig. 1, [* and ***represent p < 0.05 and p < 0.001 respectively, statistical comparisons were done by one-way analysis of variance (ANOVA) between cells transfected with HEV-Rluc RNA, and cells transfected with HEV-Rluc RNA and treated with IFN-α]. (b) Expression of ISGs; ISG56, Mda5, Mx1 and PKR genes in Huh7.5 cells was checked by SYBR-green based qRT-PCR, 24 h post RNA transfection (mRNA levels (fold) were calculated in relation to the levels of respective genes in mock transfected cells). The data represents mean ± SD of two independent triplicate sets of experiments [*represents p < 0.05, comparison between IFN-α treated cells and cells transfected with HEV-Rluc RNA and treated with IFN-α].