Table 7.
Characterization of batches of BCG-loaded “F14_Low” microparticles prepared with increasing concentrations of BCG.
| Batches | Formulation Method | BCG Pasteur Load (CFU/mL) | Particle Size, d(0.5) (µm) | Span | Zeta Potential (mV) | E.E. (%) |
|---|---|---|---|---|---|---|
| A | II | - | 60.9 ± 5.5 | 3.0 ± 5.5 | 14.1 ± 0.6 | -- |
| B | II | 1.7×106 | 35.3 ± 2.6 | 1.8 ± 0.1 | 10.5 ± 1.5 | 70.0 ± 1.6 |
| C | II | 8.3×106 | 39.3 ± 3.9 | 2.8 ± 1.8 | 12.9 ± 2.3 | 74.0 ± 3.9 |
| D | II | 1.7×107 | 41.0 ± 6.3 | 2.2 ± 0.1 | 11.8 ± 2.5 | 85.0 ± 4.8 |
| E | II | 3.3×107 | 36.8 ± 2.0 | 2.0 ± 0.2 | 13.0 ± 3.9 | 87.0 ± 3.4 |
| F | III | - | 18.2 ± 1.2 | 3.2 ± 0.7 | −3.2 ± 0.9 | -- |
| G | III | 1.7×106 | 22.2 ± 1.0 | 6.5 ± 2.6 | −16.4 ± 2.1 | -- |
| H | III | 8.3×106 | 28.3 ± 4.0 | 25.5 ± 12.3 | −12.7 ± 2.4 | 76.0 ± 1.4 |
| I | III | 1.7×107 | 22.5 ± 2.7 | 4.0 ± 2.8 | −13.7 ± 2.0 | 84.0 ± 3.4 |
| J | III | 3.3×107 | 21.4 ± 2.4 | 2.5 ± 0.4 | −12.2 ± 2.6 | 83.0 ± 10.7 |